Chromatin and DNA shearing system
A cooled, multi-sample ultrasonic system for chromatin and DNA fragmentation, processing up to 18 sealed tubes with sample volumes from 50 µL to 1 mL.
PRODUCT OVERVIEW
The Q800R product family is currently presented as the Q800R3 system. It combines high-intensity ultrasound, a rotation system, digital control and a sound-reducing enclosure for parallel processing in commercially available tubes.
The stated DNA fragment range is 150 bp to 3 kb. Multiple rack geometries accommodate different tube sizes and sample volumes. Capacity reaches 18 samples in the appropriate rack; larger tubes use racks with fewer positions.
The complete system includes a chiller, ultrasonic horn, generator, converter, rotation system, cables, tubing and three tube rack options. Programmable memory supports repeatable processing conditions, while the integrated cooling system controls temperature. The listed system warranty is two years.
CAPABILITIES
APPLICATION FIT
Processing capacity depends on sample properties, tube or vessel geometry and the selected probe or rack.
TECHNICAL DETAILS
APPLICATION REFERENCE
Reported sample conditions illustrate applications of the Q800R system. These are application reference data; results depend on the sample, tube and preparation.
| Parameter | Mammalian chromatin | Yeast chromatin | C. elegans chromatin |
|---|---|---|---|
| Sample | HEK 293T, two 10 cm dishes, 70-80% confluent | Wild-type S. pombe, OD 1.3-1.5 in YEA | C. elegans culture; nuclear extract 1 mg/mL |
| Volume | 300 µL | 300 µL | 500 µL |
| Reported fixation | 1% formaldehyde, 13 min | 1% formaldehyde, 15 min | 2% formaldehyde, 20 min |
| Amplitude | 70% | 100% | 70% |
| Pulse timing | 15 s on / 45 s off | 20 s on / 40 s off | 30 s on / 30 s off |
| Total sonication on-time | 30 min | 30 min | 20 min |
| Parameter | Bacterial genomic DNA | Human skeletal myoblast genomic DNA |
|---|---|---|
| Sample / amount | E. coli / 250 ng | LHCN-M2 skeletal myoblast / 200 ng |
| Sample volume and tube | 200 µL in 0.5 mL tube | 200 µL in 0.5 mL tube |
| Amplitude | 20% | 20% |
| Pulse timing | 15 s on / 15 s off | 15 s on / 15 s off |
| Total sonication on-time | No numerical value supplied in the product-page table | No numerical value supplied in the product-page table |
PARTS & ACCESSORIES
Rack formats include eighteen 0.3 mL thin-wall PCR tubes, twelve 0.5 mL thin-wall PCR tubes, or eight 1.5 mL polystyrene or polypropylene tubes. Two 0.5 mL rack geometries accommodate different tube lengths. Custom rack configurations are available.
Particulate filtration for the recirculating cooling water, protecting the chiller internal components.
Replacement coolant containers for the cooling system, available in packs of five.
GLEN MILLS APPLICATION SUPPORT
Talk with our team about Q800R3 Sonicator, sample volumes and available configurations.